Kali ini Rossi harus mengalah dengan Dani Pedrosa. Rider Repsol Honda ini sanggup mencatat waktu tercepat di sesi latihan kedua, MotoGP Assen mengalahkan rekan setimnya Marc Marquez. Sedangkan VR46 harus puas di posisi ketiga dengan catatan waktu 1 menit 33,652 detik, lebih lambat 0,202 detik dari Dani Pedrosa. Sedangkan, Andrea Ianonne bisa merangsek ke posisi empat,sebelumnya dia harus puas di sembilan. dan Cal Crutchlow mengikuti Ianonne dengan menempati ke lima. Jorge Lorenzo harus puas di belakang Crutchlow.
Sedangkan Marco Melandri dengan Aprilia Racing Team terpaksa menjadi juru kunci dengan perolehan waktu 1 menit 37.933 detik. (otorider.com)
1. Dani Pedrosa ESP Repsol Honda Team (RC213V) 1m 33.450s [Lap 18/20] 310km/h (Top Speed)
2. Marc Marquez ESP Repsol Honda Team (RC213V) 1m 33.589s +0.139s [5/19] 310km/h
3. Valentino Rossi ITA Movistar Yamaha MotoGP (YZR-M1) 1m 33.652s +0.202s [20/21] 306km/h
4. Andrea Iannone ITA Ducati Team (Desmosedici GP15) 1m 33.699s +0.249s [18/19] 315km/h
5. Cal Crutchlow GBR CWM LCR Honda (RC213V) 1m 33.734s +0.284s [16/19] 308km/h
6. Jorge Lorenzo ESP Movistar Yamaha MotoGP (YZR-M1) 1m 33.741s +0.291s [16/17] 308km/h
7. Andrea Dovizioso ITA Ducati Team (Desmosedici GP15) 1m 33.844s +0.394s [19/19] 313km/h
8. Aleix Espargaro ESP Team Suzuki Ecstar (GSX-RR) 1m 33.857s +0.407s [18/18] 298km/h
9. Danilo Petrucci ITA Octo Pramac Racing (Desmosedici GP14.1) 1m 34.133s +0.683s [19/20] 304km/h
10. Maverick Viñales ESP Team Suzuki Ecstar (GSX-RR)* 1m 34.232s +0.782s [18/19] 302km/h
11. Pol Espargaro ESP Monster Yamaha Tech 3 (YZR-M1) 1m 34.321s +0.871s [14/19] 308km/h
12. Yonny Hernandez COL Octo Pramac Racing (Desmosedici GP14.2) 1m 34.406s +0.956s [18/20] 306km/h
13. Bradley Smith GBR Monster Yamaha Tech 3 (YZR-M1) 1m 34.627s +1.177s [19/23] 309km/h
14. Scott Redding GBR Estrella Galicia 0,0 Marc VDS (RC213V) 1m 34.643s +1.193s [20/20] 302km/h
15. Stefan Bradl GER Athina Forward Racing (Forward Yamaha) 1m 34.664s +1.214s [18/20] 304km/h
16. Loris Baz FRA Athina Forward Racing (Forward Yamaha)* 1m 34.897s +1.447s [13/18] 300km/h
17. Eugene Laverty IRL Aspar MotoGP Team (RC213V-RS)* 1m 35.047s +1.597s [16/21] 300km/h
18. Nicky Hayden USA Aspar MotoGP Team (RC213V-RS) 1m 35.149s +1.699s [15/19] 297km/h
19. Alvaro Bautista ESP Factory Aprilia Gresini (RS-GP) 1m 35.489s +2.039s [15/18] 301km/h
20. Hector Barbera ESP Avintia Racing (Desmosedici GP14 Open) 1m 35.538s +2.088s [20/20] 309km/h
21. Jack Miller AUS CWM LCR Honda (RC213V-RS)* 1m 35.646s +2.196s [14/19] 300km/h
22. Mike Di Meglio FRA Avintia Racing (Desmosedici GP14 Open) 1m 35.663s +2.213s [15/17] 305km/h
23. Alex De Angelis RSM E-Motion IodaRacing (ART) 1m 36.869s +3.419s [12/21] 291km/h
24. Marco Melandri ITA Factory Aprilia Gresini (RS-GP) 1m 37.225s +3.775s [17/17] 300km/h