Valentino Rossi tampak pede dengan sasis baru yang membuat dia kembali ke posisi pertama. dengan torehan waktu 1 menit 34,357. Sedangkan Marquez yang sempat terjatuh harus puas di posisi kedua dengan waktu 1 menit 34,552, selisih 0,195 detik dari VR46. Sedangkan Dani Pedrosa berada di posisi ketiga diikuti Jorge Lorenzo.
Sedangkan, Maverick Vinales harus puas di posisi tujuh bersama rekan setimnya, Aleix Espargaro. Uniknya, skuad Ducati belum bisa menyodok dan hanya mampu bertengger di posisi 9 dengan Andrea Dovizioso. Pembalap Karel Abraham harus absen di Assen, karena cedera kaki yang dialaminya saat melakukan sesi test di Catalunya.
Hasil Free Practice 1 MotoGP Assen Belanda :
1. Valentino Rossi ITA Movistar Yamaha MotoGP (YZR-M1) 1m 34.357s [Lap 20/20] 302km/h (Top Speed)
2. Marc Marquez ESP Repsol Honda Team (RC213V) 1m 34.552s +0.195s [17/20] 308km/h
3. Dani Pedrosa ESP Repsol Honda Team (RC213V) 1m 34.570s +0.213s [19/19] 309km/h
4. Jorge Lorenzo ESP Movistar Yamaha MotoGP (YZR-M1) 1m 34.772s +0.415s [18/18] 306km/h
5. Pol Espargaro ESP Monster Yamaha Tech 3 (YZR-M1) 1m 34.927s +0.570s [21/21] 307km/h
6. Bradley Smith GBR Monster Yamaha Tech 3 (YZR-M1) 1m 35.042s +0.685s [21/21] 310km/h
7. Maverick Viñales ESP Team Suzuki Ecstar (GSX-RR)* 1m 35.050s +0.693s [18/19] 303km/h
8. Aleix Espargaro ESP Team Suzuki Ecstar (GSX-RR) 1m 35.175s +0.818s [15/17] 299km/h
9. Andrea Dovizioso ITA Ducati Team (Desmosedici GP15) 1m 35.223s +0.866s [13/13] 315km/h
10. Danilo Petrucci ITA Octo Pramac Racing (Desmosedici GP14.1) 1m 35.271s +0.914s [7/21] 304km/h
11. Jack Miller AUS CWM LCR Honda (RC213V-RS)* 1m 35.348s +0.991s [16/18] 298km/h
12. Cal Crutchlow GBR CWM LCR Honda (RC213V) 1m 35.393s +1.036s [17/19] 305km/h
13. Andrea Iannone ITA Ducati Team (Desmosedici GP15) 1m 35.400s +1.043s [12/20] 312km/h
14. Stefan Bradl GER Athina Forward Racing (Forward Yamaha) 1m 35.486s +1.129s [20/20] 303km/h
15. Yonny Hernandez COL Octo Pramac Racing (Desmosedici GP14.2) 1m 35.924s +1.567s [14/15] 303km/h
16. Nicky Hayden USA Aspar MotoGP Team (RC213V-RS) 1m 35.965s +1.608s [14/15] 297km/h
17. Scott Redding GBR Estrella Galicia 0,0 Marc VDS (RC213V) 1m 36.158s +1.801s [12/21] 303km/h
18. Eugene Laverty IRL Aspar MotoGP Team (RC213V-RS)* 1m 36.223s +1.866s [18/22] 299km/h
19. Hector Barbera ESP Avintia Racing (Desmosedici GP14 Open) 1m 36.233s +1.876s [15/16] 307km/h
20. Alvaro Bautista ESP Factory Aprilia Gresini (RS-GP) 1m 36.250s +1.893s [16/18] 302km/h
21. Loris Baz FRA Athina Forward Racing (Forward Yamaha)* 1m 36.423s +2.066s [16/18] 299km/h
22. Mike Di Meglio FRA Avintia Racing (Desmosedici GP14 Open) 1m 36.619s +2.262s [15/18] 303km/h
23. Alex De Angelis RSM E-Motion IodaRacing (ART) 1m 37.486s +3.129s [9/18] 293km/h
24. Marco Melandri ITA Factory Aprilia Gresini (RS-GP) 1m 37.933s +3.576s [16/17] 296km/h
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